BD LSRFortessaTM Filters Features The standard configuration of the BD LSRFortessa™ can be changed in order to detect fluorophores with emission peaks not covered by the standard filter set. In the following list you can see all additionally available filters with some fluorophores, covered better by the alternative filter…

Filter 670/30 730/45 780/60 APC Alexa 700 APC-Cy7 Alexa 633 APC-Alexa 750 Alexa 635 APC-H7 (BD) Alexa 647 APC-eFluor 780 (eBioscience) Cy5 To-Pro-3 LIVE/DEAD Fixable Far Red (Invit.) eFluor 660 (eBio.) Vibrant DyeCycle Ruby (Invit.) Fortessa Configuration

• 5 lasers Specific to a set of lasers and filters. • Refer to Jul 9, 2016 such as BD cytometers, FACSCalibur, LSRII, Fortessa, and Canto, require the pressurized If so, you will need to bleed any air out of filters. Collec on Filter. Fluorochromes (Some). 355nM.

PE-Cy5 VPD450. Pacific Blue™. BD LSRFortessa™ and. The BD LSRFortessa™ cell analyzer offers the ultimate in choice for flow cytometry, providing power, performance, and consistency.

It houses 5 lasers and has a similar configuration as our BD Fortessa. fluorescence detectors with optical filters optimized for the detection of FITC, PE, PerCP, BD LSRFortessa™ X-20. BD FA. CSAria™ Product Family.

The LSR Fortessa has a control panel with three speeds: Low (~12 µl/min), Medium (~35 µl/min) and High (~60 µl/min). There is also a sample fine rate knob to the left. The LSR-Fortessa can handle over 10,000 events a second without loss of accuracy. Sample Injection Port (SIP):(Tube support arm) SIP has two positions.

Group User and Password 5) If working with bacteria, open the black nob in front of the FSC. 6) Open the optical filter compartment (on the left side of the Fortessa and two small ports in front) to make sure that the filters necessary for detecting your fluorophores are in place. Fortessa behind right door above control panel) via the silicon tube to the sample needle and pull the syringe plunger.

BD Accuri™ C6 BD FACSCalibur™ BD FACSVerse™* BD FACSCanto™ II BD LSRFortessa™ X-20 BD FACSAria™ Product Family BD Influx™ BD FACSJazz™ Excitation Laser Line Filter Relative Brightness Violet Laser (405 nm) •••••• 405 nm 450/40 BD Horizon™ …

YFP. GFP, FITC, Alexa Du behöver ditt gamla filter och en linjal eller tumstock. Matcha sedan diametern på bottenanslutningen, filtrets längd och diameter med filtren i listan nedan så har du hittat rätt filter. Har du ett bad från Svenska Badrum eller Swebad så är stigningen på gängan annorlunda så läs på artikelkortet och välj för dessa tillverkare. The LSR Fortessa has a control panel with three speeds: Low (~12 µl/min), Medium (~35 µl/min) and High (~60 µl/min). There is also a sample fine rate knob to the left. The LSR-Fortessa can handle over 10,000 events a second without loss of accuracy. Sample Injection Port (SIP):(Tube support arm) SIP has two positions.

Alexa 405. eFluor 450 prior to running an experiment! *Denotes filter change. Aug 7, 2019 LSR Fortessa. Analyzer.
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BD Horizon PE- CF594. 610/20. PE-Cy5 VPD450. Pacific Blue™. BD LSRFortessa™ and.

o Perform a prime and install a tube with FACSClean. If you are getting events continue washing with FACSClean BD, BD Logo and all other trademarks are the property of Becton, Dickinson and Company.
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The BD LSR Fortessa™ X-20 cell analyzer delivers high-performance multicolor analysis detecting up to 18 parameters simultaneously with using 5 lasers: UV (355 nm) Violet (405 nm) Blue (488 nm) Y-G (561 nm) Red (640 nm) Fortessa X20 Flow Cytometer Quick guide Operation of the 5 laser Fortessa X20 BD Webinar CS&T System BD Compensation Guide…

Please see the separate filter guide for information on dye and filter options. Performance Fluorescence sensitivity FITC: 80 molecules of equivalent soluble fluorochrome (MESF-FITC) Filter 670/30 730/45 780/60 APC Alexa 700 APC-Cy7 Alexa 633 APC-Alexa 750 Alexa 635 APC-H7 (BD) Alexa 647 APC-eFluor 780 (eBioscience) Cy5 To-Pro-3 LIVE/DEAD Fixable Far Red (Invit.) eFluor 660 (eBio.) Vibrant DyeCycle Ruby (Invit.) Fortessa Configuration • Log in to BD FACSDiva software MACHINE SETUP: • Turn on the machine if you are the first user of the day. There is no fluidics startup for the Fortessa. • Give the lasers 20-30 minutes of warm-up time. • Check tank levels for FACS buffer and waste o To fill FACS buffer unscrew probe and place in holster.

Each signal is directed through an array of mirrors and filters to a specific detector . Fortessa X20 5-18; Fortessa 4-15; Fortessa 4-15 HTS; Attune NxT 4-14; Accuri C6; LSRII 4-12. Model : LSR Fortessa X20 Manufacturer: BD Biosciences

2013-06-27 · With the industry's most advanced high throughput plate loader and hotel, the S1000 family of analyzers go head-to-head with BD FACS Canto, BD LSR II, and BD Fortessa at a fraction of the cost and size while offering a superior solution for conventional and high throughput flow cytometry applications. To help with pinpointing exact data, the analyzer has the capacity to zoom in, allowing you easily visualize spectra intersection points with laser lines, filter sets and other spectra. To zoom in, double click your mouse over the area that you wish to zoom, or roll the mouse wheel forward. Catalog Number BD LSRFortessa X-20 This product is no longer available on Biocompare. Biocompare is the leading resource for up-to-date product information, product reviews, and new technologies for life scientists.

Checking Cytometer performance 1.